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Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
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生物工程
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JoVE 杂志 生物工程
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

DOI:
10.3791/62599-v

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12:18 min

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August 03, 2021

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, ,
1Department of Chemical Engineering,Worcester Polytechnic Institute, 2Center for Biomedical Engineering, School of Engineering,Brown University

Chapters

  • 00:04Introduction
  • 00:33Developing and Characterizing Uni-Lipid Vesicles
  • 04:33Forming a Uni-Lipid Supported Lipid Bilayer Using QCM-D
  • 07:29Forming a Suspended Lipid Bilayer
  • 08:05Developing Multi-Lipid Cell Mimicking Vesicles and Bilayers
  • 08:48Molecule Interaction Studies with Uni-Lipid and Multi-Lipid Supported Bilayers
  • 09:29Molecule Interaction Studies with Uni-Lipid and Multi-Lipid Suspended Bilayers
  • 10:15Results: Development of Uni- and Multi-Lipid Cell Mimicking Vesicles and Bilayers
  • 11:51Conclusion

Summary

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This protocol describes the formation of cell mimicking uni-lipid and multi-lipid vesicles, supported lipid bilayers, and suspended lipid bilayers. These in vitro models can be adapted to incorporate a variety of lipid types and can be used to investigate various molecule and macromolecule interactions.

Tags

Keywords: Lipid BilayerMembrane InteractionsPharmaceutical AgentsEnvironmental ToxicantsBiological CompoundsVesicle SuspensionExtrusionPolycarbonate MembraneSupported Lipid BilayerSuspended Lipid Bilayer

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