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Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

4,418 Views

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12:26 min

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February 12, 2022

DOI:

10.3791/62589-v

DOI:
10.3791/62589-v

•

12:26 min
February 12, 2022

•

11 Views
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1Helmholtz Institute for RNA-based Infection Research (HIRI),Helmholtz Zentrum für Infektionsforschung (Helmholtz Centre for Infection Research), 2Medical Faculty,Julius-Maximilians University Würzburg

Tags

Optical TweezersRNA-protein InteractionsTranslation RegulationSingle Molecule TechniquesFluorescence Assisted Optical TweezersForce-ramp ExperimentsConstant Force MeasurementsReal-time VisualizationDNA-RNA ConstructIn-vitro TranscriptionPCR Reactions

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Cite this Article

Pekarek, L., Buck, S., Caliskan, N. Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation. J. Vis. Exp. (180), e62589, doi:10.3791/62589 (2022).

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