Journal
/
生物学
/
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp
JoVE 杂志
生物学
Author Produced
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物学
Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

Staining the Cytoplasmic Ca2+ with Fluo-4/AM in Apple Pulp

DOI:
10.3791/62526-v

•

08:05 min

•

November 06, 2021

•

, , ,
1College of Horticulture,Qingdao Agricultural University

Chapters

  • 00:03Introduction
  • 00:57Protoplast Extraction
  • 01:49Small-molecule Calcium Ion Fluorescence Staining
  • 04:46Protoplast Viability Assay
  • 05:48Results
  • 07:21Conclusion

Summary

自动翻译

自动翻译

Isolated protoplasts of apple pulp cells were loaded with a calcium fluorescent reagent to detect cytoplasmic Ca2+ concentration.

Tags

Keywords: Cytoplasmic Ca2+Fluo-4/AMApple PulpCalcium ImagingProtoplast ExtractionSmall Molecule Fluorescent IndicatorCalcium Ion SelectivityEsterification IncubationEnzymatic SolutionFluorescence Microscope

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

check_url/cn/62526?article_type=v

Focal Ca2+ Transient Detection in Smooth Muscle
check_url/cn/62526?article_type=v

In vivo Ca2+– Imaging of Mushroom Body Neurons During Olfactory Learning in the Honey Bee
check_url/cn/62526?article_type=v

Techniques for Imaging Ca2+ Signaling in Human Sperm
check_url/cn/62526?article_type=v

Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
check_url/cn/62526?article_type=v

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
check_url/cn/62526?article_type=v

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
check_url/cn/62526?article_type=v

Automated Analysis of Dynamic Ca2+ Signals in Image Sequences
check_url/cn/62526?article_type=v

Stimulation of Cytoplasmic DNA Sensing Pathways In Vitro and In Vivo
check_url/cn/62526?article_type=v

Imaging Local Ca2+ Signals in Cultured Mammalian Cells
check_url/cn/62526?article_type=v

Methods to Classify Cytoplasmic Foci as Mammalian Stress Granules

Read Article