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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

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14:48 min

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May 08, 2021

DOI:

10.3791/62512-v

DOI:
10.3791/62512-v

•

14:48 min
May 08, 2021

•

6 Views
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1Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine,Magna Graecia University of Catanzaro, 2Department of Health Science,Magna Graecia University of Catanzaro, 3Romolo Hospital, 4Department of Experimental and Clinical Medicine,Magna Graecia University of Catanzaro

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Proteomic ProfileEPS-urineFASP DigestionData-independent AnalysisMass SpectrometryBiomarker DiscoveryProstate CancerCentrifugationSample PreparationProtein AlkylationUrea BufferIodoacetamide

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Prestagiacomo, L. E., Gabriele, C., Morelli, P., Rota, M. A., Alba, S., Cuda, G., Damiano, R., Gaspari, M. Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis. J. Vis. Exp. (171), e62512, doi:10.3791/62512 (2021).

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