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Investigating the Phagocytosis of Leishmania using Confocal Microscopy
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免疫与感染
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JoVE 杂志 免疫与感染
Investigating the Phagocytosis of Leishmania using Confocal Microscopy

Investigating the Phagocytosis of Leishmania using Confocal Microscopy

DOI:
10.3791/62459-v

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08:41 min

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July 29, 2021

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, , , , , ,
1Laboratory of Host-Parasite Interaction and Epidemiology,Gonçalo Moniz Institute, 2National Institute of Science and Technology of Tropical Diseases – National Council for Scientific Research and Development (CNPq)

Chapters

  • 00:05Introduction
  • 01:20Leishmania Phagocytosis Assessment by Macrophages
  • 02:50Evaluation of Leishmania‐Induced Vacuole Maturation
  • 04:55Confocal Microscopy Acquisition and Quantification
  • 06:08Results: Assessment of Leishmania spp. Phagocytosis
  • 07:43Conclusion

Summary

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The mechanism associated with phagocytosis in Leishmania infection remains poorly understood. Here, we describe methods to evaluate the early events occurring during Leishmania interaction with the host cells.

Tags

Keywords: PhagocytosisLeishmaniaConfocal MicroscopyTHP-1 CellsMacrophagesPromastigotesPV BiogenesisTransfection

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