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Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
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JoVE 杂志 免疫与感染
Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

DOI:
10.3791/62436-v

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15:01 min

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September 18, 2021

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, , , , , ,
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases,Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry,Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry),Forschungszentrum Jülich

Chapters

  • 00:04Introduction
  • 01:03Aspergillus fumigatus Conidia Application & Specimen Preparation
  • 01:22Mouse Lung Lobe Optical Clearing
  • 02:23Mouse Lung Lobe Imaging with CLSM
  • 06:50Image Processing: Surface Rendering
  • 09:23Image Processing: Mask Correction
  • 12:54Conidia Quantitative Analysis
  • 13:41Representative Results
  • 14:07Conclusions

Summary

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We describe the method for quantitative analysis of the distribution of Aspergillus fumigatus conidia (3 µm in size) in the airways of mice. The method also can be used for the analysis of microparticles and nanoparticle agglomerate distribution in the airways in various pathological condition models.

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Keywords: Confocal Laser Scanning MicroscopyAspergillus FumigatusConidiaOptical ClearingMouse LungQuantitative AnalysisRespiratory TractMucociliary ClearanceAlveolar MacrophagesNeutrophilsOptical ClearingBenzyl AlcoholBenzyl BenzoateConfocal MicroscopyLaserSpectral RangeDetector GainPinholePixel ResolutionZ-stack

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