Journal
/
遗传学
/
Genoombrede analyse van histonmodificatieverdeling met behulp van de chromatine-immunoprecipitatie-sequencingmethode in Magnaporthe oryzae
JoVE 杂志
遗传学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 遗传学
Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
Please note that all translations are automatically generated. Click here for the English version.

Genoombrede analyse van histonmodificatieverdeling met behulp van de chromatine-immunoprecipitatie-sequencingmethode in Magnaporthe oryzae

DOI:
10.3791/62423-v

•

09:25 min

•

June 02, 2021

•

, , , , , ,
1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy,Beijing University of Agriculture

Chapters

  • 00:04Introduction
  • 00:39Preparation of Protoplasts from M. oryzae
  • 01:48In Vivo Crosslinking and Sonication
  • 03:22IP of Crosslinked Protein/DNA
  • 04:23Collecting and Rinsing the IP Products
  • 05:04Elution and Reverse Crosslinking of Protein/DNA Complexes
  • 05:56Purification and Recovery of DNA
  • 06:51DNA Repair and Solexa Library Construction
  • 08:23Results: Chromatin Immunoprecipitation Sequencing Method for Genome-wide Histone Modification Analysi
  • 08:53Conclusion

Summary

自动翻译

自动翻译

Hier presenteren we een protocol om de genoombrede verdeling van histonmodificaties te analyseren, die nieuwe doelgenen in de pathogenese van M. oryzae en andere filamenteuze schimmels kunnen identificeren.

Tags

Chromatin Immunoprecipitation Sequencing (ChIP-seq)Histone ModificationsMagnaporthe OryzaeEpigenetic RegulationFungal PathogenesisGenome-wide AnalysisTranscription FactorsDNA-protein InteractionsProtoplast PreparationSonicationAgarose Gel Electrophoresis

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Genoom-brede analyse van zoogdieren Replication Timing door DNA Content Measurement

Analyse van de c-KIT Ligand Promoter Gebruik van Chromatine Immunoprecipitatie

Chromatine Immunoprecipitatie (ChIP) in Muis T-cellijnen

Generatie van inheemse chromatine Immunoprecipitation Sequencing bibliotheken nucleosoom dichtheid p.a.

Chromatine Immunoprecipitation (ChIP) Protocol voor lage-overvloed embryonale monsters

Toewijzing genoom-brede toegankelijk chromatine in primaire menselijke T lymfocyten door ATAC-Seq

Generatie van genoom-brede chromatine conformatie Capture bibliotheken uit strak geënsceneerde vroege Drosophila embryo 's

Chromatine Immunoprecipitation (ChIP) van Histone modificaties van Saccharomyces cerevisiae

Promotor Capture Hi-C: High-resolution, genoom-brede profilering van promotor interacties

Chromatine Immunoprecipitation van lymfkliertest bruin vetweefsel

Read Article