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Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research
JoVE 杂志
生物学
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JoVE 杂志 生物学
Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research

Harvesting and Disaggregation: An Overlooked Step in Biofilm Methods Research

DOI:
10.3791/62390-v

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13:25 min

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April 22, 2022

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, , , , , ,
1Center for Biofilm Engineering,Montana State University

Chapters

  • 00:47Introduction
  • 01:53Vortexing and Sonication
  • 04:29Scraping and Homogenization
  • 07:31Scraping, Vortexing, and Sonication
  • 09:48Results
  • 12:16Conclusion

Summary

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This paper details methods that demonstrate three common biofilm harvesting and disaggregation techniques on two surface types, ruggedness testing of a harvesting method and minimum information to consider when choosing and optimizing harvesting and disaggregation techniques to increase reproducibility.

Tags

Keywords: BiofilmHarvestingDisaggregationVortexingSonicatingScrapingHomogenizingPolycarbonateBorosilicate GlassSilicone TubingCDC Biofilm ReactorDrip Flow ReactorCatheter ModelPseudomonas AeruginosaASTM StandardsSamplingAnalysis

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