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Generasjon av sebrafisk Larval Xenografts og tumor atferdsanalyse
JoVE 杂志
癌症研究
Author Produced
This content is Free Access.
JoVE 杂志 癌症研究
Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis
DOI:

12:08 min

June 19, 2021

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Chapters

  • 00:00Introduction
  • 01:37Setting Up for Injection
  • 02:0424 Hours Before Injection
  • 02:48Injection Day
  • 04:24Injection Procedures
  • 06:12Metastasic Assay
  • 07:104 Day Post-Injection
  • 08:05Whole Mount Immunostining for Confocal Imaging
  • 08:35Mounting of Xenografts
  • 09:02Confocal Imaging
  • 10:22Representative Results
  • 11:45Conclusion

Summary

自动翻译

Her tilbyr vi en trinnvis protokoll, med tips for å generere xenografter og retningslinjer for tumoradferdsanalyse, helmontert immunfluorescens og konfokal bilde kvantifisering.

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