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斑马鱼拉瓦尔异种草和肿瘤行为分析的一代
JoVE 杂志
癌症研究
Author Produced
This content is Free Access.
JoVE 杂志 癌症研究
Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis
DOI:

12:08 min

June 19, 2021

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Chapters

  • 00:00Introduction
  • 01:37Setting Up for Injection
  • 02:0424 Hours Before Injection
  • 02:48Injection Day
  • 04:24Injection Procedures
  • 06:12Metastasic Assay
  • 07:104 Day Post-Injection
  • 08:05Whole Mount Immunostining for Confocal Imaging
  • 08:35Mounting of Xenografts
  • 09:02Confocal Imaging
  • 10:22Representative Results
  • 11:45Conclusion

Summary

自动翻译

在这里,我们提供一个分步协议,为肿瘤行为分析、全山免疫荧光和共焦成像定量提供生成异种移植物的提示和指南。

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