The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and timescales.
Nawijn, C., Segers, T., Lajoinie, G., Mørch, Ý., Berg, S., Snipstad, S., de Lange Davies, C., Versluis, M. Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release. J. Vis. Exp. (172), e62251, doi:10.3791/62251 (2021).