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Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies
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JoVE 杂志 免疫与感染
Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

DOI:
10.3791/62214-v

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08:37 min

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February 15, 2021

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, , ,
1Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, 2Vita-Salute San Raffaele University

Chapters

  • 00:04Introduction
  • 00:39Spleen Processing
  • 01:29T Cell Enrichment
  • 03:27iNKT Cell Enrichment
  • 05:21iNKT Cell Activation and Expansion
  • 06:35Results: Enrichment, Expansion, and Characterization of iNKT Cells
  • 07:50Conclusion

Summary

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We describe a rapid and robust protocol to enrich invariant natural killer T (iNKT) cells from mouse spleen and expand them in vitro to suitable numbers for in vitro and in vivo studies.

Tags

Mouse Invariant Natural Killer T CellsPurificationExpansionIn VitroIn VivoCell IsolationCell EnrichmentMACSCell StainingCell SeparationCell Culture

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