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发育生物学
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Creating Avian Forebrain Chimeras to Assess Facial Development
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发育生物学
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JoVE 杂志 发育生物学
Creating Avian Forebrain Chimeras to Assess Facial Development

Creating Avian Forebrain Chimeras to Assess Facial Development

DOI:
10.3791/62183-v

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04:10 min

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February 18, 2021

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1Department of Orthopaedic Surgery, Orthopaedic Trauma Institute,University of California

Chapters

  • 00:05Introduction
  • 00:40Preparing the Donor Tissue
  • 01:45Preparing the Host
  • 02:36Results: Development and Assessment of Forebrain Chimeras
  • 03:36Conclusion

Summary

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This article describes a tissue transplantation technique that was designed to test the signaling and patterning properties of basal forebrain during craniofacial development.

Tags

Avian Forebrain ChimeraFacial DevelopmentBasal ForebrainNeural Crest CellsDMEM MediaNeutral RedTungsten NeedleEgg ShellEmbryoGraftHamburger-Hamilton StageQCPN AntibodyQuail-chick System

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