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CRISPR/Cas9基因组编辑的半球 藻（ Hübner）的胚胎显微注射和敲除突变体鉴定
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06:37 min
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July 01, 2021
DOI:
1School of Chemical and Environmental Engineering,China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection,Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences
Summary
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这里展示的是 Helicoverpa armigera （Hübner）胚胎显微注射和通过CRISPR / Cas9基因组编辑创建的敲除突变体鉴定的方案。突变昆虫能够进一步研究体内不同基因的功能和相互作用。
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Cite this Article
Ai, D., Wang, B., Fan, Z., Fu, Y., Yu, C., Wang, G. Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner). J. Vis. Exp. (173), e62068, doi:10.3791/62068 (2021).
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