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使用 MALDI 质谱成像进行代谢分析的示例准备
JoVE 杂志
生物学
This content is Free Access.
JoVE 杂志 生物学
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
DOI:

09:08 min

December 22, 2020

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Chapters

  • 00:04Introduction
  • 00:46Tissue Preparation
  • 01:18Cryosection Acquisition
  • 03:24Matrix Preparation
  • 04:13Matrix Deposition
  • 06:16Results: Representative Output with Selected m/z Spectra from Mass Spectrometry Acquired at 50-micron Lateral Resolution
  • 07:37Conclusion

Summary

自动翻译

本协议的目标是在规划使用 MALDI MSI 进行实验时,为样品制备提供详细的指导,以最大限度地提高生物样品中的代谢和分子检测。

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