Journal
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发育生物学
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Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

DOI:
10.3791/61999-v

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08:08 min

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November 19, 2020

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, ,
1Institute of Molecular Health Sciences, Swiss Federal Institute of Technology,ETH Zurich

Chapters

  • 00:05Introduction
  • 01:09Holding and Microinjection Pipette Preparation
  • 02:58Intracytoplasmic Double Knockout Parthenogenetic Haploid Embryonic Stem Cell (DKO-phaESC) Injection
  • 05:34Results: Representative Establishment and Application of DKO-phaESC
  • 07:23Conclusion

Summary

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This article aims to demonstrate the use of parthenogenetic haploid embryonic stem cells as a substitute for sperm for the construction of semi-cloned embryos.

Tags

Mouse Parthenogenetic Haploid Embryonic Stem CellsSperm SubstituteGenomic ImprintingEmbryo GenerationGamete ManipulationMicroinjection Pipette PreparationIntracytoplasmic Injection

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