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由加德纳菌阴道膀胱暴露在小鼠中引发的复发性大肠杆菌尿路感染

JoVE 杂志
免疫与感染

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JoVE 杂志免疫与感染

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

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由加德纳菌阴道膀胱暴露在小鼠中引发的复发性大肠杆菌尿路感染

DOI:

10.3791/61967-v

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08:53 min

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December 04, 2020

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Valerie P. O’Brien^2,6, Matthew S. Joens⁷, Amanda L. Lewis^2,4,8, Nicole M. Gilbert⁵

¹Department of Molecular Microbiology,Washington University School of Medicine in Saint Louis, ²Center for Women’s Infectious Disease Research,Washington University School of Medicine in Saint Louis, ³Center for Cellular Imaging,Washington University School of Medicine in Saint Louis, ⁴Department of Obstetrics and Gynecology,Washington University School of Medicine in Saint Louis, ⁵Department of Pediatrics,Washington University School of Medicine in Saint Louis, ⁶Fred Hutchinson Cancer Research Center, ⁷TESCAN USA, Inc., ⁸University of California San Diego

Chapters

00:05Introduction
00:41Bladder Exposures to G. vaginalis
01:53Urine Cytology
02:31Imaging Bladders by Scanning Electron Microscopy
06:23Results: G. vaginalis Triggers UPEC rUTI
08:27Conclusion

Summary

自动翻译

展示了产尿道 大肠杆菌 （UPEC）经尿道接种以建立潜伏细胞内膀胱储液囊的小鼠模型，随后膀胱暴露于 阴道 G. 以诱导复发性 UPEC UTI。还证明了细菌的计数，尿液细胞学检查以及原位膀胱固定和扫描电子显微镜处理。

Tags

Recurrent UTI Escherichia Coli Gardnerella Vaginalis Bladder Exposure Mouse Model Urogenital Bacteria Bladder Exfoliation Anaerobic Culture Bacterial Inoculum Urine Analysis Cytospin PMNs Epithelial Cells Catheterization Bladder Exposure

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