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Cocultura de neuronas ganglionas retinal de rata axotomizada con glia olfativa, como modelo in vitro de regeneración axonal adulta
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神经科学
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JoVE 杂志 神经科学
Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration
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Cocultura de neuronas ganglionas retinal de rata axotomizada con glia olfativa, como modelo in vitro de regeneración axonal adulta

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07:57 min

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November 02, 2020

DOI:

10.3791/61863-v

DOI:
10.3791/61863-v

•

07:57 min
November 02, 2020

•

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1Facultad de CC Experimentales,Universidad Francisco de Vitoria, 2Dept. Biomedical and Biotechnological Sciences, Section of Physiology,University of Catania, 3Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina,Universidad Autónoma de Madrid

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AxotomizedRetinal Ganglion NeuronsOlfactory Ensheathing GliaIn VitroAxonal RegenerationCocultureQuantitative TechniqueCell TherapyNervous System InjuriesRetinal DissectionPapainDNaseCentrifugationOvo Mucoid Protease InhibitorNB-B27 MediumPLL-treated CoverslipsOEG Monolayer

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Portela-Lomba, M., Simón, D., Russo, C., Sierra, J., Moreno-Flores, M. T. Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration. J. Vis. Exp. (165), e61863, doi:10.3791/61863 (2020).

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