Here we present a hydrophobic tissue clearing method that allows for the viewing of target molecules as part of intact brain structures. This technique has now been validated for F344/N control and HIV-1 transgenic rats of both sexes.
Kirchner, K. N., Li, H., Denton, A. R., Harrod, S. B., Mactutus, C. F., Booze, R. M. A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (166), e61821, doi:10.3791/61821 (2020).