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A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis
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JoVE 杂志 免疫与感染
A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

DOI:
10.3791/61808-v

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06:30 min

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January 27, 2021

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, , , , ,
1College of Veterinary Medicine,Yangzhou University, 2Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, 3Department of Animal Husbandry and Veterinary Medicine,Beijing Agricultural Vocational College

Chapters

  • 00:04Introduction
  • 00:40Construction of the MicC Deletion Mutant
  • 04:11Results: PCR Verification and Virulence Properties of the 50336△micC Mutants
  • 06:04Conclusion

Summary

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An λ-Red-mediated recombination system was used to create a deletion mutant of a small non-coding RNA micC.

Tags

Homologous RecombinationGene InactivationVirulence GenesAttenuated MutantSalmonella EnteritidisChloramphenicol CassetteMicC Gene

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