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High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence
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JoVE 杂志 免疫与感染
High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

DOI:
10.3791/61597-v

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07:25 min

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February 23, 2021

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,
1Department of Chemical and Biomolecular Engineering,University of Houston

Chapters

  • 00:05Introduction
  • 00:33Microarray Plate Screening
  • 03:26Validating the Identified Conditions
  • 05:30Results: Microarray and Validation
  • 06:36Conclusion

Summary

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In this method paper, we present a high-throughput screening strategy to identify chemical compounds, such as osmolytes, that have a significant impact on bacterial persistence.

Tags

High-throughput ScreeningBacterial PersistenceChemical CompoundsDrug PanelsGene LibrariesMicroarrayOfloxacinPersister AssayColony Forming Units (CFUs)Serial DilutionOrbital ShakerModified LB Medium

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