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Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
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JoVE 杂志 免疫与感染
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice

Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice

DOI:
10.3791/61535-v

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05:39 min

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July 08, 2020

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, , , , , , , ,
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 2Division of Intensive Care Unit, First Department of Integrated Medicine, Saitama Medical Center,Jichi Medical University, 3Department of Medical Physiology,Meiji Pharmaceutical University

Chapters

  • 00:04Introduction
  • 00:43Obtaining Kidney Samples from HIGA Mice
  • 01:27Total RNA Purification from Kidney Samples
  • 02:14cDNA Synthesis and qRT‐PCR of miRNA
  • 03:55Results: Expression Levels of miRNAs in the Kidneys of HIGA Mice
  • 04:57Conclusion

Summary

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自动翻译

microRNAs are involved in the pathogenesis of IgA nephropathy. We have developed a reliable method for detecting microRNA expression levels in the kidneys of an IgA nephropathy mouse model (HIGA mice). This new method will facilitate to check for miRNAs involvement in IgA nephropathy.

Tags

MicroRNAIGA NephropathyKidneyMouse ModelQRT-PCRRNA IsolationCDNA Synthesis

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