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发育生物学
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Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

Whole Animal Imaging of Drosophila melanogaster using Microcomputed Tomography

DOI:
10.3791/61515-v

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10:36 min

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September 02, 2020

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1Department of Molecular Biology,University of Wyoming

Chapters

  • 00:05Introduction
  • 00:44Sample Selection, Cuticle Preparation, Fixation, and Staining
  • 02:52Sample Mounting
  • 03:46Scanning
  • 05:51Reconstruction and Image Analysis
  • 08:07Results: Representative Drosophila melanogaster Imaging
  • 09:56Conclusion

Summary

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A protocol is presented that allows for the visualization of intact Drosophila melanogaster at any stage of development using microcomputed tomography.

Tags

Drosophila MelanogasterMicrocomputed TomographyWhole Animal ImagingNon-destructive ImagingDevelopmental DefectsDisease ModelingSample PreparationBouin SolutionPBSStainingCritical Point DryingSample Mounting

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