This content is Free Access.
Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry
3,096 Views
•
08:07 min
•
May 19, 2020
DOI:
Summary
Automatically generated
This protocol describes a method for culturing primary hippocampal neurons from embryonic mouse brain. The expression of major histocompatibility complex class I on the extracellular surface of cultured neurons is then assessed by flow cytometric analysis.
Read Article
Cite this Article
Funk, K. E., Lotz, S. K. Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry. J. Vis. Exp. (159), e61436, doi:10.3791/61436 (2020).
Copy