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JoVE Journal Developmental Biology
Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice
 

Real-Time Imaging of CCL5-Induced Migration of Periosteal Skeletal Stem Cells in Mice

Article DOI: 10.3791/61162-v • 06:10 min • September 16th, 2020
September 16th, 2020 •
1,2, 1, 1, 1,2,3
1Department of Molecular & Human Genetics, Baylor College of Medicine, 2Center for Skeletal Biology, Baylor College of Medicine, 3Department of Pathology & Immunology, Baylor College of Medicine

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This protocol describes the detection of CCL5-mediated periosteal skeletal stem cell migration in real-time using live animal intravital microscopy.

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Real-time Imaging CCL5-induced Migration Periosteal Skeletal Stem Cells Mice Standardized Protocol Endogenous Cell Migration Skeletal Phenotypes In Vivo Cell Migration Individual Cells Injury Cytokine Treatment Toe Pinch Response Ointment Application Transverse Incision Forceps Periosteum Separation Calvaria Periosteum Sterile Cotton Swab Skin Flap Folding Sagittal Sutures Coronal Sutures Sterile PBS Flushing Bevel Needle Placement
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