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Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses
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JoVE 杂志 免疫与感染
Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

Mouse Footpad Inoculation Model to Study Viral-Induced Neuroinflammatory Responses

DOI:
10.3791/61121-v

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09:07 min

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June 14, 2020

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, ,
1Department of Molecular Biology,Princeton University, 2Princeton Neuroscience Institute,Princeton University

Chapters

  • 00:04Introduction
  • 00:38Mouse Footpad Abrasion
  • 01:15Pseudorabies Virus (PRV) Footpad Inoculation
  • 01:58Tissue Collection
  • 03:32Spinal Cord and Dorsal Root Ganglion (DRG) Extraction
  • 05:49Tissue Homogenization
  • 06:51Results: Representative Viral-Induced Neuroinflammatory Response Evaluation
  • 08:37Conclusion

Summary

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The footpad inoculation model is a valuable tool for characterizing viral-induced neuroinflammatory responses in vivo. In particular, it provides a clear assessment of viral kinetics and associated immunopathological processes initiated in the peripheral nervous system.

Tags

Mouse FootpadViral InfectionNeuroinflammatory ResponsePeripheral Nervous SystemCentral Nervous SystemC57BL/6 MouseAbrasionVirus InoculationOrgan HarvestingBrain Extraction

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