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In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems
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JoVE 杂志 环境科学
In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

In Situ Chemotaxis Assay to Examine Microbial Behavior in Aquatic Ecosystems

DOI:
10.3791/61062-v

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07:23 min

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May 05, 2020

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, , , ,
1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering,ETH Zürich, 2Climate Change Cluster,University of Technology Sydney, 3School of Oceanography,University of Washington

Chapters

  • 00:04Introduction
  • 00:51Chemical of Interest Preparation and In Situ Chemotaxis Assay (ISCA) Filling for Lab Deployment
  • 01:49Lab Deployment
  • 02:37Laboratory Sample Retrieval
  • 03:09Field Chemoattractant Preparation
  • 04:12Field Deployment
  • 05:55Results: Representative In Vitro and Field Microbial Chemotaxis Analyses
  • 06:41Conclusion

Summary

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Presented here is the protocol for an in situ chemotaxis assay, a recently developed microfluidic device that enables studies of microbial behavior directly in the environment.

Tags

Keywords: In Situ Chemotaxis AssayISCAMicrobial BehaviorAquatic EcosystemsChemoattractantBacterial CultureFlow CytometryQuantificationMicrobial FunctionEnvironmental Sampling

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