需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
6,776 Views
•
09:01 min
•
November 17, 2020
DOI:
1Translational Medicine Research Center, Ruijin Hospital North,Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology,Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation,Shanghai Jiao Tong University School of Medicine
Summary
Automatically generated
This manuscript presents a protocol to induce active experimental autoimmune encephalomyelitis (EAE) in mice. A method for the isolation and characterization of the infiltrated lymphocytes in the central nervous system (CNS) is also presented to show how lymphocytes are involved in the development of CNS autoimmune disease.
Read Article
Cite this Article
Ji, Z., Zhou, C., Niu, H., Wang, J., Shen, L. Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis. J. Vis. Exp. (165), e61050, doi:10.3791/61050 (2020).
Copy