Journal
/
免疫与感染
/
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

DOI:
10.3791/61039-v

•

11:40 min

•

April 17, 2020

•

, , ,
1Department of Biology,Indiana University

Chapters

  • 00:04Introduction
  • 00:32Generation of Recombinant Virus
  • 04:00Plaque Isolation of Recombinant Viruses
  • 07:02Gel Electrophoresis of Viral dsRNA
  • 09:11Results: Characteristics of the Recombinant Strains rSA11/wt and rSA11/NSP3-2A-3xFL-UnaG
  • 10:58Conclusion

Summary

自动翻译

自动翻译

Generation of recombinant rotaviruses from plasmid DNA provides an essential tool for the study of rotavirus replication and pathogenesis, and the development of rotavirus expression vectors and vaccines. Herein, we describe a simplified reverse genetics approach for generating recombinant rotaviruses, including strains expressing fluorescent reporter proteins.

Tags

Reverse GeneticsRecombinant RotavirusReporter ProteinsBHKT7 CellsMA104 CellsTransfectionTrypsinPlasmid MixtureFluorescent Marker Proteins

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

‘Bioluminescent’ Reporter Phage for the Detection of Category A Bacterial Pathogens

A Method for Labeling Vasculature in Embryonic Mice

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Reverse Genetics Mediated Recovery of Infectious Murine Norovirus

Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System

Using Fluorescent Proteins to Monitor Glycosome Dynamics in the African Trypanosome

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

Targeting Cysteine Thiols for in Vitro Site-specific Glycosylation of Recombinant Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Read Article