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Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C)

6,301 Views

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10:02 min

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April 29, 2020

DOI:

10.3791/60960-v

DOI:
10.3791/60960-v

•

10:02 min
April 29, 2020

•

1 Views
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1Center for Genomic Regulation (CRG),The Barcelona Institute of Science and Technology, 2Universitat Pompeu Fabra, 3Vall d’Hebron Institute of Oncology

Summary

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We report the application of quantitative chromosome conformation capture followed by high-throughput sequencing in embryoid bodies generated from embryonic stem cells. This technique allows to identify and quantitate the contacts between putative enhancers and promoter regions of a given gene during embryonic stem cell differentiation.

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4CChromosome Conformation CaptureEnhancer-promoter ContactsEmbryoid BodiesMouse Embryonic Stem CellsGene RegulationCis-regulatory ElementsChromatin InteractionsCell CultureCell Differentiation

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Cite this Article

Tian, T. V., Vidal, E., Graf, T., Stik, G. Identification of Enhancer-Promoter Contacts in Embryoid Bodies by Quantitative Chromosome Conformation Capture (4C). J. Vis. Exp. (158), e60960, doi:10.3791/60960 (2020).

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