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在果蝇黑色无gaster的Axon死亡期间，对Axon及其突触的形态和功能评估
JoVE 杂志
神经科学
Author Produced
This content is Free Access.
JoVE 杂志 神经科学
Morphological and Functional Evaluation of Axons and their Synapses during Axon Death in Drosophila melanogaster
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在果蝇黑色无gaster的Axon死亡期间，对Axon及其突触的形态和功能评估

DOI:
10.3791/60865-v

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10:29 min

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March 16, 2020

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, , ,
1Department of Fundamental Neurosciences,University of Lausanne

Chapters

  • 00:05Introduction
  • 01:28Observation of Axon Morphology During Axon Death in the PNS (Peripheral Nervous System)
  • 03:22Observation of Axon and Synapse Morphology During Axon Death in the CNS (Central Nervous System)
  • 05:53Grooming Induced by Optogenetics as a Readout for Axon and Synapse Function
  • 07:56Results: Axon Death of GFP-Labeled Sensory Neuron Axons and Axonal/Synaptic Function After Axotomy
  • 09:40Conclusion

Summary

自动翻译

自动翻译

在这里，我们提供协议，在果蝇黑色素气仪中执行三个简单的伤害诱发的斧子退化（斧子死亡）测定，以评估被切断的斧子及其突触的形态和功能保存。

Tags

Drosophila MelanogasterAxon DegenerationAxon MorphologySynapse FunctionPartial Wing InjuryAntennal AblationOptogeneticsNeuronal Maintenance FactorsAxonal TransportAxonal OrganellesSpinning Disk Microscopy

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