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神经科学
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Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

13,662 Views

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10:05 min

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May 23, 2020

DOI:

10.3791/60822-v

DOI:
10.3791/60822-v

•

10:05 min
May 23, 2020

•

43 Views
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1Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina,Universidade de Lisboa, 2Instituto de Farmacologia e Neurociências, Faculdade de Medicina,Universidade de Lisboa, 3Instituto de Biologia Molecular, Faculdade de Medicina,Universidade de Lisboa

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In this article, we describe, in detail, a protocol for the generation of neurosphere cultures from postnatal mouse neural stem cells derived from the main mouse neurogenic niches. Neurospheres are used to identify neural stem cells from brain tissue allowing the estimation of precursor cell numbers. Moreover, these 3D structures can be plated in differentiative conditions, giving rise to neurons, oligodendrocytes and astrocytes, allowing the study of cell fate.

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Cite this Article

Soares, R., Ribeiro, F. F., Lourenço, D. M., Rodrigues, R. S., Moreira, J. B., Sebastião, A. M., Morais, V. A., Xapelli, S. Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches. J. Vis. Exp. (159), e60822, doi:10.3791/60822 (2020).

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