Here, we present a protocol to conduct a variety of behavioral tests in cattle that have been designed to evaluate emotions. A battery of behavioral tests (open field test, startle test, bovine zero maze, exit velocity, pen score, and chute score) were conducted to evaluate different components of animal temperament.
Daigle, C. L., Hubbard, A. J., Grandin, T. The Use of Traditional Fear Tests to Evaluate Different Emotional Circuits in Cattle. J. Vis. Exp. (158), e60641, doi:10.3791/60641 (2020).