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Immunology and Infection

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JoVE Journal Immunology and Infection
NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
 

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

Article DOI: 10.3791/60567-v • 10:57 min • January 12th, 2020
January 12th, 2020 •
1, 1
1Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus

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Here, we present a protocol to quickly and easily measure nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation in cell lines expressing NF-κB::luciferase reporter constructs, via measurements of luminescence in the cell lysate. Additionally, gene expression is determined via RT-qPCR isolated from cells infected with Salmonella Typhimurium.

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NF-kappa-B-dependent Luciferase Activation Quantification Gene Expression Salmonella Infected Tissue Culture Cells NF-kappa-B Reporter High-throughput Screen Transcription Factor Inflammatory Responses Cytokines Chemokines HeLa Cell Line NF-kappa-B Induction Salmonella Typhimurium Bacteria Viruses Chemical Compounds Cell Lines Sterile Technique Cell Culture Bacterial Work
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