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发育生物学
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Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish
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发育生物学
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JoVE 杂志 发育生物学
Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish

Displacement Analysis of Myocardial Mechanical Deformation (DIAMOND) Reveals Segmental Heterogeneity of Cardiac Function in Embryonic Zebrafish

DOI:
10.3791/60547-v

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09:15 min

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February 06, 2020

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1Division of Cardiology, Department of Medicine,David Geffen School of Medicine at University of California, Los Angeles, 2Ronald Reagan UCLA Medical Center, 3Veterans Affairs West Los Angeles Medical Center

Chapters

  • 00:04Introduction
  • 01:13Reconstruction of the 3D Systolic and Diastolic Heart and Segmentation of the Ventricle
  • 02:41Creation of Rectangular Parallelepipeds for Image Registration and Resampling of Systolic and Diastolic 3D Hearts Along the Short Axis Plane
  • 05:01Division of the Resampled Heart
  • 06:20Registration of Systolic and Diastolic Image Matrices and Output of Displacement Vectors
  • 07:01Results: Segmental Heterogeneity in Cardiac Function and Susceptibility to Chemotherapy-induced Injury
  • 08:45Conclusion

Summary

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The goal of this protocol is to detail a novel method for the assessment of segmental cardiac function in embryonic zebrafish under both physiological and pathological conditions.

Tags

– Displacement Analysis Of Myocardial Mechanical Deformation (DIAMOND)- Segmental Cardiac Function- Zebrafish Embryos- Quantitative Assessment- Four-dimensional- Cardiac Mechanics- Doxorubicin-induced Cardiac Toxicity- High-throughput In Vivo Screening- Mammalian Heart- Muscle Fiber Orientation- Rotational And Twisting Motion- Anatomical Structure- Ventricle Segmentation- 3D TIF Files- Image Analysis Software- Prepimage 1.m

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