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Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach
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化学
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JoVE 杂志 化学
Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum through a Chemometric Approach

DOI:
10.3791/60186-v

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09:32 min

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September 26, 2019

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, , , , , , ,
1Procter and Gamble,Beijing Innovative center, 2Procter and Gamble,Mason Business Center, 3Department of Dermatology,Beijing Children’s Hospital, 4Chinese Academy of Inspection and Quarantine

Chapters

  • 00:04Title
  • 01:22Collection of In Vivo Confocal Raman Spectra from Human Subjects with Atopic Dermatitis
  • 02:10Removal of the Out-of-skin Spectra
  • 04:38Removing the Outlier Spectra with the Room Light Effect
  • 06:16Selection of the Number of Components in MCR Decomposition Analysis
  • 07:35Results: Resolving Water, Proteins, and Lipids from In Vivo Confocal Raman Spectra of Stratum Corneum
  • 08:56Conclusion

Summary

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Here, we present a protocol for collection of confocal Raman spectra from human subjects in clinical studies combined with chemometric approaches for spectral outlier removal and the subsequent extraction of key features.

Tags

Keywords: In Vivo Confocal Raman SpectroscopyStratum CorneumWaterProteinsLipidsChemometricsData PreprocessingBaseline CorrectionOutlier DetectionDepth Profiling

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