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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应
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生物化学
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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应

9,116 Views

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10:28 min

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August 17, 2019

DOI:

10.3791/60017-v

DOI:
10.3791/60017-v

•

10:28 min
August 17, 2019

•

13 Views
, , , ,
1Department of Molecular Medicine,The Scripps Research Institute, 2Department of Neuroscience,The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI,National Institutes of Health

Summary

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一种烟酰胺腺苷二核苷酸（NADH）与ATPase的检测已应用于小分子肌苷抑制剂的半高通量筛选。此动力学测定以 384 孔微孔板格式运行，总反应体积仅为每孔 20 μL。该平台应适用于几乎任何ADP生产酶。

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Cite this Article

Radnai, L., Stremel, R. F., Sellers, J. R., Rumbaugh, G., Miller, C. A. A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors. J. Vis. Exp. (150), e60017, doi:10.3791/60017 (2019).

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