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Isolation, Transfection, and Culture of Primary Human Monocytes
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JoVE 杂志 免疫与感染
Isolation, Transfection, and Culture of Primary Human Monocytes
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Isolation, Transfection, and Culture of Primary Human Monocytes

DOI:
10.3791/59967-v

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09:13 min

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December 16, 2019

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, , , , ,
1Department of Medicine,Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Chapters

  • 00:05Title
  • 00:53Primary Human Monocyte Isolation
  • 03:22Primary Human Monocyte Culture
  • 04:25Primary Human Monocyte Transfection
  • 05:53M1/M2 Differentiation and Activation
  • 07:07Results: Representative Transfection Efficiency, Viability, and Protein Regulation Effectivity
  • 08:35Conclusion

Summary

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Presented here is an optimized protocol for isolating, culturing, transfecting, and differentiating human primary monocytes from HIV-infected individuals and healthy controls.

Tags

Primary Human MonocytesIsolationTransfectionCultureHIV InfectionImmune DysfunctionAntiretroviral TherapyMolecular MechanismsBiosafetyEDTAMagnetic BeadsPBSCentrifugationCell Counting

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