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生物化学
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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
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生物化学
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JoVE 杂志 生物化学
Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

DOI:
10.3791/59942-v

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05:47 min

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February 19, 2020

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, ,
1Department of Anatomy and Cell Biology,University of Yamanashi School of Medicine, 2Shimadzu Corporation

Chapters

  • 00:04Title
  • 01:09Solid Tissue and Body Fluids Preparation
  • 03:43Results: Mass Spectra
  • 04:52Conclusion

Summary

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This article introduces sample preparation methods for a unique real-time analytical method based on the ambient mass spectrometry. This method lets us perform real-time analysis of the biological molecules in vivo without any special pretreatments.

Tags

Probe Electrospray IonizationMass SpectrometryBiological SamplesIn VitroIn VivoCell SortingFractionationConcentrationSilicaProliferatePESI-MSMolecular IdentityLiverKidneyScalpelTrepanPhosphate-buffered SalineHomogenizeEthanolIonization ChamberDirect Probe Ionization Mass SpectrometerStainless Steal Needle ProbeElectrosprayMass Spectra

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