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Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies
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JoVE 杂志 免疫与感染
Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

6,612 Views

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10:59 min

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June 01, 2019

DOI:

10.3791/59899-v

DOI:
10.3791/59899-v

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10:59 min
June 01, 2019

•

3 Views
, , , , ,
1Post-Graduate Program in Veterinary Sciences,Federal Rural University of Rio de Janeiro, 2Department of Animal Parasitology,Federal Rural University of Rio de Janeiro, 3Department of Epidemiology and Public Health,Federal Rural University of Rio de Janeiro

Summary

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Analysis of tick hemolymph represents an important source of information on how some pathogens cause disease and how ticks immunologically respond to this infection. The present study demonstrates how to inoculate fungal propagules and collect hemolymph from Rhipicephalus microplus engorged females.

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TickRhipicephalus MicroplusHemolymph CollectionMetarhizium BlastosporesInoculationEntomopathogenic FungiArthropod Immune ResponseHumoral ResponseCellular ResponseHemocytesPathogen InoculationArthropod Pathology

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Fiorotti, J., Gôlo, P. S., Marciano, A. F., Camargo, M. G., Angelo, I. C., Bittencourt, V. R. E. P. Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies. J. Vis. Exp. (148), e59899, doi:10.3791/59899 (2019).

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