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长距离反向PCR对热线1的逆变活性的检测

JoVE 杂志
癌症研究
Author Produced

This content is Free Access.

JoVE 杂志癌症研究

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

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长距离反向PCR对热线1的逆变活性的检测

8,255 Views

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10:54 min

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July 27, 2019

DOI:

10.3791/59880-v

DOI:

10.3791/59880-v

•

10:54 min

July 27, 2019

•

1 Views

Barun Pradhan^1,2, Liisa Kauppi^1,2

¹Systems Oncology Research Program,University of Helsinki, ²Department of Biochemistry and Developmental Biology, Medicum,University of Helsinki

Summary

本文概述了一种基于PCR的简单测定,用于监测活性LINE-1逆转录的活性,并绘制给定基因组中反逆转位图。使用 MCF7 细胞系,我们在此演示如何应用此方法来检测位于 22q12.1 的 LINE-1 的活动。

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