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In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
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Cancer Research
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JoVE Journal Cancer Research
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

DOI:
10.3791/59849-v

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11:07 min

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August 14, 2019

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, ,
1Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland

Chapters

  • 00:06Title
  • 00:44Preparation of Embryos for Implantation
  • 01:31Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
  • 03:55Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
  • 08:34Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
  • 10:37Conclusion

Summary

Automatic Translation

Automatic Translation

The aim of this method is to generate an in vivo model of tumor angiogenesis by xenografting mammalian tumor cells into a zebrafish embryo that has fluorescently-labelled blood vessels. By imaging the xenograft and associated vessels, a quantitative measurement of the angiogenic response can be obtained.

Tags

Keywords: In Vivo ImagingTumor XenograftsZebrafishAngiogenesisVascularizationQuantitationB16-F1 CellsMicroinjectionTransgenic EmbryosECMYolk Sac

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