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Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification
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09:17 min
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July 12, 2019
DOI:
1Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 2Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University, 3Department of Neurosurgery,University of Mississippi Medical Center
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Here, we present a protocol of adult mouse terminal phalanx amputation to investigate mammalian blastema formation and intramembranous ossification, analyzed by fluorescent immunohistochemistry and sequential in-vivo microcomputed tomography.
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Dawson, L. A., Brunauer, R., Zimmel, K. N., Qureshi, O., Falck, A. R., Kim, P., Dolan, C. P., Yu, L., Lin, Y., Daniel, B., Yan, M., Muneoka, K. Adult Mouse Digit Amputation and Regeneration: A Simple Model to Investigate Mammalian Blastema Formation and Intramembranous Ossification. J. Vis. Exp. (149), e59749, doi:10.3791/59749 (2019).
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