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发育生物学
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Injecting Gryllus bimaculatus Eggs
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发育生物学
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JoVE 杂志 发育生物学
Injecting Gryllus bimaculatus Eggs

Injecting Gryllus bimaculatus Eggs

DOI:
10.3791/59726-v

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00:08 min

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August 22, 2019

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, , , , ,
1Colby College, 2Division of Evolutionary Developmental Biology,National Institute for Basic Biology, 3Department of Biological Sciences,National University of Singapore, 4Department Biology and Department of Neuroscience,Bowdoin College, 5Department of Organismic and Evolutionary Biology and Department of Molecular and Cellular Biology,Harvard University

Chapters

  • 00:04Title
  • 00:32Making Needles for Injection
  • 01:27Egg Collection and Preparation
  • 03:55Injection
  • 06:29Results: Analysis of the Injected Gryllus bimaculatus Eggs
  • 08:00Conclusion

Summary

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Here we present a protocol to inject cricket eggs, a technique which serves as a foundational method in many experiments in the cricket, including, but not limited to, RNA interference and genomic manipulation.

Tags

Gryllus BimaculatusCricket Embryonic DevelopmentRNA InterferenceGenomic ManipulationEgg InjectionBeveled NeedleAgarose Well PlateEgg Collection DishOviposition

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