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Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
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JoVE 杂志 免疫与感染
Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

DOI:
10.3791/59725-v

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00:06 min

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August 23, 2019

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, , , , , , ,
1Key Laboratory of Comprehensive Utilization of Advantage Plants Resources in Hunan South, College of Chemistry and Bioengineering,Hunan University of Science and Engineering, 2College of Food and Biological Engineering,Zhejiang Gongshang University

Chapters

  • 00:04Title
  • 00:29Preparation of Fermentation Medium and Human Fecal Sample
  • 01:48In Vitro Batch Fermentation
  • 02:33EPS Degradation by Human Fecal Microbiota
  • 03:29Effects of EPS on SCFA (Short-chain Fatty Acids) Production by Human Intestinal Microbiota
  • 04:41Results: Degradation by Fermentation
  • 06:24Conclusion

Summary

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Described here is a protocol to investigate the interactions between endobiotics and human gut microbiota using in vitro batch fermentation systems.

Tags

In VitroGut MicrobiotaEndobioticsFermentationPolysaccharideShort-chain Fatty AcidTLCOrcinolHigh-throughput ScreeningDiagnosis

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