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Neuroscience

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JoVE Journal Neuroscience
Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke
 

Using Zebrafish Larvae to Study the Pathological Consequences of Hemorrhagic Stroke

Article DOI: 10.3791/59716-v • 06:36 min • June 5th, 2019
June 5th, 2019 •
1, 2, 2,3, 1,3, 1,3
1Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Manchester Academic Health Science Centre, University of Manchester, 2Division of Cardiovascular Sciences, School of Medical Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, 3Lydia Becker Institute of Immunology and Inflammation, University of Manchester

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Here we present a protocol to quantify brain injury, locomotor deficits and neuroinflammation following bleeding in the brain in zebrafish larvae, in the context of human intracerebral hemorrhage (ICH).

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Zebrafish Larvae Pathological Consequences Hemorrhagic Stroke Pre-clinical Approach Cellular Response Blood In The Brain Specific Medications Transparency Of Zebrafish Larvae Live Intact Animals Fluorescent Microscopy Tea Strainer Fertilized Embryos Breeding Boxes Petri Dish E3 Embryo Medium Incubate At 28 Degrees Celsius Stage According To Standard Guidelines Bright-field Stereo Microscope Ultra Thin Dissection Forceps Atorvastatin Treatment Control Dish Larvae Hemorrhaged
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