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用于狂犬病诊断的泛源病毒实时RT-PCR
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免疫与感染
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JoVE 杂志 免疫与感染
Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis
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用于狂犬病诊断的泛源病毒实时RT-PCR

20,701 Views

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06:25 min

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July 10, 2019

DOI:

10.3791/59709-v

DOI:
10.3791/59709-v

•

06:25 min
July 10, 2019

•

18 Views
, , , , , ,
1Wildlife Zoonoses & Vector-Borne Diseases Research Group,Animal and Plant Health Agency, 2Institute of Infection and Global Health,University of Liverpool

Summary

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这种使用dsDNA间染的实时RT-PCR适用于诊断裂解病毒感染。该方法从从狂犬病疑似前身或死后样本中提取的RNA开始,详细说明主混合物制备、RNA添加、实时机器的设置以及结果的正确解释。

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Cite this Article

Marston, D. A., Jennings, D. L., MacLaren, N. C., Dorey-Robinson, D., Fooks, A. R., Banyard, A. C., McElhinney, L. M. Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis. J. Vis. Exp. (149), e59709, doi:10.3791/59709 (2019).

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