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Generation af inducerede neurale stamceller fra perifere mononukleære celler og differentiering mod dopaminerge neuron-prækursorer til transplantations studier
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发育生物学
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JoVE 杂志 发育生物学
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies

Generation af inducerede neurale stamceller fra perifere mononukleære celler og differentiering mod dopaminerge neuron-prækursorer til transplantations studier

DOI:
10.3791/59690-v

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12:13 min

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July 11, 2019

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,
1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, 2Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

Chapters

  • 00:04Title
  • 01:31Isolation of PBMNCs (Peripheral Blood Mononuclear Cells)
  • 03:00Expansion of MNCs
  • 04:12Reprogramming of PBMNCs to iNSCs (Induced Neural Stem Cells) by SeV (Sendai Virus) Infection
  • 07:20Establishment of Unilateral 6-Hyroxydopamine (6-OHDA)-Lesioned Parkinson s Disease (PD) Mouse Models
  • 09:15Results: Induced Neural Stem Cells Differentiation Toward Dopaminergic Neuron Precursors and Treatment of Parkinson s Disease Mouse Models
  • 11:00Conclusion

Summary

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自动翻译

Protokollen præsenterer omprogrammering af mononukleære celler i perifert blod for at inducere neurale stamceller ved Sendai-virus infektion, differentiering af iNSCs i dopaminerge neuroner, transplantation af DA-prækursorer til den ensidigt læsionerede Parkinsons sygdom-musemodeller og evaluering af sikkerheden og effekten af iNSC-afledte DA-prækursorer til PD-behandling.

Tags

Induced Neural Stem CellsPeripheral Mononuclear CellsDopaminergic Neuron PrecursorsParkinson’s DiseaseAutologous Cell SourceProliferative CapacityRegion-specific Neural CellsNeurological DiseasesDensity Gradient MediumMononuclear CellsCell ExpansionCell FreezingCell Counting

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