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从外周单核细胞生成诱导神经干细胞,分化至多巴胺能神经元前体,用于移植研究
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JoVE 杂志 发育生物学
Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron Precursors for Transplantation Studies
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从外周单核细胞生成诱导神经干细胞,分化至多巴胺能神经元前体,用于移植研究

DOI:
10.3791/59690-v

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12:13 min

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July 11, 2019

•

,
1Cell Therapy Center, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, and Key Laboratory of Neurodegeneration,Ministry of Education, 2Center of Neural Injury and Repair,Beijing Institute for Brain Disorders

Chapters

  • 00:04Title
  • 01:31Isolation of PBMNCs (Peripheral Blood Mononuclear Cells)
  • 03:00Expansion of MNCs
  • 04:12Reprogramming of PBMNCs to iNSCs (Induced Neural Stem Cells) by SeV (Sendai Virus) Infection
  • 07:20Establishment of Unilateral 6-Hyroxydopamine (6-OHDA)-Lesioned Parkinson s Disease (PD) Mouse Models
  • 09:15Results: Induced Neural Stem Cells Differentiation Toward Dopaminergic Neuron Precursors and Treatment of Parkinson s Disease Mouse Models
  • 11:00Conclusion

Summary

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自动翻译

该协议提出了外周血单核细胞的重新编程,以诱导神经干细胞由仙台病毒感染,将iNSCs分化为多巴胺能神经元,将DA前体移植到单方面病变帕金森病小鼠模型,并评价iNSC衍生DA前体用于PD治疗的安全性和有效性。

Tags

Induced Neural Stem CellsPeripheral Mononuclear CellsDopaminergic Neuron PrecursorsParkinson’s DiseaseAutologous Cell SourceProliferative CapacityRegion-specific Neural CellsNeurological DiseasesDensity Gradient MediumMononuclear CellsCell ExpansionCell FreezingCell Counting

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