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転写におけるタンパク質とタンパク質の相互作用を研究するための生体層干渉計(BLI)の応用
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生物化学
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JoVE 杂志 生物化学
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
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転写におけるタンパク質とタンパク質の相互作用を研究するための生体層干渉計(BLI)の応用

12,944 Views

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07:18 min

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July 26, 2019

DOI:

10.3791/59687-v

DOI:
10.3791/59687-v

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07:18 min
July 26, 2019

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3 Views
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1Department of Pharmacology, Robert Wood Johnson Medical School,Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies,Rutgers University, 3Department of Plant Biology, School of Environmental and Biological,Rutgers University

Tags

Biolayer InterferometryBLIProtein-protein InteractionsTranscriptionKinetic ParametersBiosensorNickel NTAReal-time Association And DissociationQuantitative AnalysisPor Dialysis

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Desai, M., Di, R., Fan, H. Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription. J. Vis. Exp. (149), e59687, doi:10.3791/59687 (2019).

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